BE288BE292ComplexBermanLabs

-- BobVitale? - 17 Oct 2007

Berman Lab Complex (BE-288 & 292)

Table of Contents

Infrastructure and Equipment

C02 Gas System Distribution

C02 is distributed to the BE-288 lab from equipment room BE-286. A four cylinder gas manifold system in BE-288 is used to supply CO2 at xxpsi to the distribution system. The gas manifold system has automatic cut-over from 'empty' cylinder side to full cylinder side. Each side has 2 cyclinders. Switchover should occur when cylinder pressure falls below 170psi. When this occurs and one side has pressure lower than xxpsi, an audible alarm should sound indicating a need to replace C02 cylinders.

Praxair contact is: Luis Martinez. Gas manifold system was purchased from Praxair. Sales representative is Luis Martinez.

Distribution points in BE-288 consist of xxin nozzle outlets at the following locations. Most are used to supply C02 gas to current and future incubators.

Note on C02 quality: PBSci stockroom carries lab grade CO2. Some incubator manufacturers recommended or require medical grade C02. Reference Shel Lab C02 incubator Model 3507 manual.

Incubator Systems

A stacked incubator system (1 on top of another) are used in the lab. As of 10/17/07, the lab had one stack unit (2 incubators). Eventual build out is anticipated at 4 stacks or 8 incubators. Each incubator requires 115VAC, 4A, single phase power with generator backup. Generator backup is needed for times when power is out to building.

Incubator Manufacturer/Model: Sanyo CO2 Incubator, Model MCO-17A1 Power Required: 115VAC, 3.7A max per unit. C02 Gas Required:

Incubator Stack #1:
S/N: 80806132
S/N: 80806133

Modifications Required

Gas Modifications

• BermanLabSpecs-070727.pdf: Plumbing Mods (Gas, CO2 & Vac) for Berman Labs
  

Natural gas is needed at each island bench (in 288 & 292) and at a location near the Bio Safety cabinets in 288.
Bio saftey cabinets need vacuum connections. C02 is needed for 6-8 incubators in 288. C02 is needed for BSC.

Electrical Modifications

email from Randy 8/16/07
Bob, I checked on amperage of the incubators in Rm 288. They draw 3.7A max, which is 22.2A total for all six. I then talked to the electrician working on Rm 288. He will (1) install 2 circuits instead of one and (2) provide a fourplex outlet where the four incubators will be located.

Randy

• Electrical
• Plumbing
• Equipment -- additional lab table needed for incubator against south wall of 288.
• Consummables
• Ceiling Tiles - Replacement with Washable type. CFR (#58561) is to replace ceiling tile in Berman Tissue Culture lab with washable type. On Friday 4/20, Randy told Phil the expected cost was approximately $6,400, but the price quote came in higher at $6,941. Status: 4/24- David Bornstein sent request to Prof Berman for approval.

Dear Kathryn, Randy, and Bob,

I'm writing to summarize the brief discussion I had with Randy and Bob Vitale yesterday and to tell you of my thoughts regarding the completion of our labs in Baskin Engineering. While the original plan was move our equipment there sometime this week, we must now delay the move until our labs are finished.

Because of problems with dust and particulates, we cannot move until four main areas of construction are complete and the workman are out of the space. These areas are:

1) Electrical: where new outlets to match our equipment need to be installed (my understanding is that this process in underway and should be completed shortly);

2) Plumbing where: a) natural gas lines need to be installed in both labs, b)oxygen (Correction: CO2) lines need to be installed in the cell culture lab; and c) the biosafety hoods need to be connected to the vacuum and gas lines (CORRECTION: no gas to BSCs), and d) the MilliQue? water system is installed

3) Removal of cabinetry and shelving , patching and repainting the wall, and plumbing, in order to install the biosafety cabinents in the cell culture lab.

4) Air balancing and replacement of all ceiling tiles.

Hopefully these tasks can be completed in a matter of weeks not months, but some of the jobs such as installation of gas lines still need a scope of work written and bidding from outside contractors, and probably value engineering decisions.

In view of these delays in occupancy and the availability of funding there are two additional improvements I would like Randy to investigate both in terms of price and time to completion [Phil has startup funds remaining that he would use to cover cost]

1) Replacing the porous ceiling tiles in the tissue culture lab with vinyl (washable) ceiling tiles, and 2) Repainting the blue walls in both labs to match the beige color on the walls in the rest of the labs.

In order to facilitate tracking progress on these activities, Bob suggested setting up a 'Wiki' web site that can serve as a central point of information where we can track the progress on these activities. Hopefully this will save time and improve coordination. We also need someone in facilities to provide a draft timeline so that we can establish a new target date for occupancy.

Please let me know your thoughts on this and any items that I may have left of the list that are critical for us to occupy these labs.

Sincerely,

Phil